• 电话:0531-88364464

  • 传真:0531-88564464

  • 地址:
    中国山东省济南市山大南路27号
  • 邮编:250100



导师风采

【个人简介】

姜玮,男,1962年生人,1984年7月毕业于山东大学化学系,获理学学士学位并留校任教,分别于1992年和2003年在山东大学获理学硕士、博士学位。现任山东大学化学与化工学院教授,博士生导师,学院党委书记,院学术委员会委员,山东大学基础学部学术委员会委员,山东省化学化工学会常务理事,中国化学会会员,中国化学会有机分析专业委员会委员。

0531-88363888(O),wjiang@sdu.edu.cn 

【学习及工作经历】

1984.07 - 1991.12 山东大学化学系,助教

1991.12 - 1997.09 山东大学化学与化工学院,讲师

1997.09 - 2002.09 山东大学化学与化工学院,副教授

2002.09 - 现在 山东大学化学与化工学院,教授

2006.06 - 现在 山东大学化学与化工学院,教授,博士生导师

1999.11 - 2003.12 山东大学化学与化工学院,副院长

2004.01 - 2007.12 山东大学研究生院,副院长

2004.06 - 2007.12 兼任山东大学威海分校研究生处,处长

2007.12 - 2013.01 山东大学化学与化工学院,党委书记兼副院长

2013.01 - 现在 山东大学化学与化工学院,党委书记

【主讲课程】

分析化学前沿,分析化学,无机及分析化学,分析化学实验,仪器分析实验

【研究领域和兴趣】

研究领域为生物分析化学,研究工作集中在:
1、DNA纳米材料的设计、制备、表征与功能化;
2、多模态、多维度荧光显微成像技术与单分子检测、单细胞分析;
3、新型荧光信号放大策略与生物分子超灵敏检测技术;
4、新型纳米荧光生物传感器的设计、构建与生物医学应用。
作为项目负责人承担国家自然科学基金面上项目4项,山东省自然科学基金重点项目2项。在Analytical Chemistry,Biomaterials,Chemical Communications,Biosensor and Bioelectronics,ACS Applied Materials & Interfaces,Bioconjugate Chemistry等国际知名学术杂志发表SCI收录研究论文80余篇。

【主要论著】

70.Model-guided interface probe arrangement for sensitive protein detection
Analytical Chemstry, 2016, Accepted Manuscript
69.Application of an ultrahigh-performance liquid chromatography coupled to quadrupole-Orbitrap high-resolution mass spectrometry for the rapid screening, identification and quantification of illegal adulterated glucocorticoids in herbal medicines
Journal of Chromatography B, 2016, Accepted Manuscript
68.Target-controlled gating liposome "off–on"cascade amplification for sensitive and accurate detection of phospholipase D in breast cancer cells with a low-background signal
Chemical Communications, 2016, 52, 10660--10663
67.Toehold-mediated strand displacement reaction-dependent fluorescent strategy for sensitive detection of uracil-DNA glycosylase activity
Biosensors and Bioelectronics, 2016, Accepted Manuscript
66.Colocalization recognition-activated cascade signal amplification strategy for ultrasensitive detection of transcription factors
Biosensors and Bioelectronics, 2016, Accepted Manuscript
65.Binding Induced Colocalization Activated Hybridization Chain Reaction on the Surface of Magnetic Nanobead for Sensitive Detection of Adenosine
Biosensors and Bioelectronics, 2016, Accepted Manuscript
64.A split recognition mode combined with cascade signal amplification strategy for highly specific, sensitive detection of microRNA
Biosensors and Bioelectronics, 2016, Accepted Manuscript
63.A bicyclo-hairpin probe mediated strand displacement amplification strategy for label-free and sensitive detection of bleomycin
Sensors and Actuators B, 2017, 238, 318–324
62.T-Hg2+-T metallo-base pair-mediated dual amplification fluorescent strategy for the selective and sensitive detection of Hg2+
RSC Advances, 2016, Accepted Manuscript
61.Protein binding-protected DNA three-way junction-mediated rolling circle amplification for sensitive and specific detection of transcription factors
RSC Advances, 2016, 6, 68846–68851
60.Modular nuclease-responsive DNA three-way junctions-based dynamic assembly of DNA device and its sensing application
Analytical Chemistry, 2016, 88, 3817−3825
59.A unique dual recognition hairpin probe mediated fluorescent amplification method for sensitive detection of uracil-DNA glycosylase and endonuclease IV activities
Analyst,2016, 141, 1789–1795
58.Au Nanoparticles Fluorescence Switch-mediated Target Recycling Amplification Strategy for Sensitive Nucleic Acid Detection
RSC Advances, 2016, 6, 10650–10654
57.Cascade Signal Amplification Based on Copper Nanoparticle-Reported Rolling Circle Amplification for Ultrasensitive Electrochemical Detection of the Prostate Cancer Biomarker
ACS Appl. Mater. Interfaces 2016, 8, 2573−2581
56.Multiplexed detection of cytokines based on dual bar-code strategy and single-molecule counting
Analytical Chemistry, 2016, 88, 1578−1584
55.Target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy for the sensitive assay of DNA methyltransferase activity
Chemical Communications, 2016, 52, 2517--2520
54.A dual amplification fluorescent strategy for sensitive detection of DNA methyltransferase activity based on strand displacement amplification and DNAzyme amplification
Biosensors and Bioelectronics, 2016, 77, 650–655
53.Label-free and enzyme-free detection of transcription factors with graphene oxide fluorescence switch-based multifunctional G-quadruplex-hairpin probe
Biosensors and Bioelectronics,2016, 75, 155–160
52.Label-free detection of nicotinamide adenine dinucleotide based on ligation-triggered exonuclease III-assisted signal amplification
RSC Advances, 2015, 5, 86625–86630
51.Label-free Molecular Beacons-Based Quadratic Isothermal exponential Amplification: A simple and Sensitive One-Pot Method to Detection DNA Methyltransferase Activity
Chemical Communications, 2015, 51, 13538 - 13541
50.Hairpin assembly circuit-based fluorescence cooperative amplification strategy for enzyme-free and label-free detection of small molecule
Talanta, 2015, 143: 101–106
49.FokI cleavage–inhibition strategy for the specific and accurate detection of transcription factors
Talanta, 2015,144, 44–50
48.Label-free and dual-amplified detection of protein via small molecule-ligand linked DNA and a cooperative DNA machine
Biosensors and Bioelectronics, 2015, 72, 107–113
47.Highly selective and sensitive detection of miRNA based on toehold-mediated strand displacement reaction and DNA tetrahedron substrate
Biosensors and Bioelectronics, 2015, 71, 401–406
46.Label-free fluorescence dual-amplified detection of adenosine based on exonucleaseIII-assisted DNA cycling and hybridization chain reaction
Biosensors and Bioelectronics, 2015, 70, 15–20
45.Label-free Nucleic Acid Detection Based on DNA Templated Silver Nanoclusters Fluorescent Probe
Talanta, 2015, 138, 163–168
44.A DNA machine-based fluorescence amplification strategy for sensitive detection of uracil-DNA glycosylase activity
Biosensors and Bioelectronics, 2015, 68, 654-659
43.A Binding-induced Sutured Toehold Activation for Controllable DNA Strand Displacement Reactions
Chemical Communications, 2015, 51, 2903-2906
42.Sensitive detection of transcription factors with Ag+-stabilized self-assembly triplex DNA molecular switch
Chemical Communications, 2014, 50, 14987-14990
41.Visualizing the endocytosis of phenylephrine in living cells by quantum dot-based tracking
Biomaterials, 2014, 35, 7042-7049
40.Quantitative detection of tumor necrosis factor-α by single molecule counting based on hybridization chain reaction
Biosensors and Bioelectronics, 2014, 60, 180-184
39.Toehold-mediated Strand Displacement Reaction Triggered Isothermal DNA Amplification for Highly Sensitive and Selective Fluorescent Detection of Single-base Mutation
Biosensors and Bioelectronics, 2014, 59, 276-281
38.Cascade Signal Amplification Strategy for Sensitive and Label-free DNA Detection Based on Exo III-catalyzed Recycling Coupled with Rolling Circle Amplification
Analyst, 2014, 139, 2884-2889
37.Aptamer-Based Exonuclease Protection and Enzymatic Recycling Cleavage Amplification Homogeneous Assay for Highly sensitive Detection of thrombin
Analyst, 2014, 139, 3167-3173
36.Quantitative Single-molecule Detection of Protein Based on DNA Tetrahedron Fluorescent Nanolabels
Talanta, 2014, 125, 393-399
35.An ultrasensitive fluorescence assay for protein detection by hybridization chain reaction-based DNA nanotags
Biosensors and Bioelectronics, 2014, 51, 421-425
34.Surface-enhanced Raman spectroscopy detection of polybrominated diphenylethers using a portable Raman spectrometer
Talanta, 2013, 116, 14-17
33.Cysteamine-Modified Silver Nanoparticle Aggregates for Quantitative SERS Sensing of Pentachlorophenol with a Portable Raman Spectrometer
ACS Appl. Mater. Interfaces, 2013, 5 (15), 6902-6908
32.Cysteine-Modulated Colorimetric Sensing of Extracellular Mg2+ in Rat Brain Based on the Strong Chelation Interaction between Dithiothreitol and Mg2+
Analyst, 2013,138, 3046-3052
31.A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle amplification-responsive G-quadruplex formation for highly sensitive and selective detection of NAD+ or ATP
Chemical Communications, 2013, 49, 2640-2642
30.A Novel Enzyme-free and Label-free Fluorescence Aptasensor for Amplified Detection of Adenosine
Biosensors and Bioelectronics, 2013, 44, 52-56
29.Selective and Sensitive Mercuric (II) Ion Detection Based on Quantum Dots and Nicking Endonuclease Assisted Signal Amplification
Biosensors and Bioelectronics, 2013, 43, 84-87
28.Electrospun porous CuO-Ag nanofibers for quantitative sensitive SERS detection
CrystEngComm, 2013, 15, 1339-1344
27.Visualization the Intracellular Location and Stability of DNA origami with a Label-free Fluorescent Probe
Chemical Communications, 2012, 48, 11301-11303
26.Quantitative Detection of Single DNA Molecules on DNA Tetrahedron Decorated Substrates
Chemical Communications, 2012, 48, 9661-9663
25.In situ fabrication of single-crystalline porous ZnO nanoplates on zinc foil to support silver nanoparticles as a stable SERS substrate
CrystEngComm, 2012, 14, 6023-6028
24.UV Photolysis-induced Generation of Graphene/M (M=Ag/Au) Hybrid Nanomaterials
Materials Letters, 2012, 84, 120-123
23.Silver nanoparticle aggregates on copper foil for reliable quantitative SERS analtsis of polycyclic aromatic hydrocarbons with a portable Raman spectrometer
Analyst, 2012, 137, 3995-4000
22.Micrococcal Nuclease Detection Based on Peptide-Bridged Energy Transfer between Quantum Dots and Dye-Labeled DNA
Talanta, 2012, 97, 533-538
21.Sensitive Detection of Proteins Using Assembled Cascade Fluorescent DNA Nanotags Based on Rolling Circle Amplication
Bioconjugate Chemistry, 2012, 23, 734-739
20.Strong Interaction between Imidazolium-Based Polycationic Polymer and Ferricyanide: toward Redox Potential Regulation for Selective In Vivo Electrochemical Measurements
Analytical Chemistry, 2012, 84, 1900-1906
19.A versatile platform for highly sensitive detection of protein: DNA enriching magnetic nanoparticles based rolling circle amplication immunoassay
Chemical Communications, 2012, 33, 3930-3932. Inside cover paper
18.Synthesis of a quinazoline derivative: A new a 1-adrenoceptors ligand for conjugation to quantum dots to study a 1-adrenoceptors in living cells
Bioorganic & Medicinal Chemistry Letters, 2011, 21, 5905-5909
17.Label free DNA detection based on gold nanoparticles quenching fluorescence of Rhodamine B
Talanta, 2011, 85, 725-729
16.A Sensitive Enzymatic Method for Paraoxon Detection based on Enzyme Inhibition and Fluorescence Quenching
Talanta, 2011, 84, 400-405
15.Quantitative Detection of Single Molecules Using Enhancement of Dye/DNA Conjugate-Labeled Nanoparticles
Bioconjugate Chemistry, 2010, 21, 1987-1993
14.Solid phase single-molecule counting of antibody binding to supported protein layers surface with low nonspecific adsorption
Talanta, 2010, 82, 1003-1009
13.Fluorescence single-molecule counting assays for protein quantification using epi-fluorescence microscopy with quantum dots labeling
Analytical Chimica Acta, 2010, 662, 170-176
12.DNA Quantification Based on FRET Realized by Combination with Surfactant CPB
Talanta, 2010, 81, 597-601
11.The co-luminescence effect of a europium(III)-lanthanum(III)-gatifloxacin-sodium dodecylbenzene sulfonate system and its application for the determination of trace amount of europium(III)
Journal of Luminescence, 2010, 130, 591-597
10.Quantitative detection of antibody based on single-molecule counting by total internal reflection fluorescence microscopy with quantum dot labeling
Analytica Chimica Acta, 2009, 634, 83-88
09.Rapid Determination of Minocycline in Pharmaceutical Formulations and Human Urine/Serum Samples Based on the Minocycline-Europium-Sodium Dodecylbenzene Sulfonate Luminescence System
Analytical Letters, 2009, 42, 228-242
08.Luminescence enhancement effect for the determination of balofloxacin with balofloxacin–europium (III)–sodium dodecylbenzene sulfonate system
Journal of Luminescence, 2009, 129, 90-94
07.Micelle-enhanced and terbium-sensitized spectrofluorimetric determination of gatifloxacin and its interaction mechanism
Spectrochimica Acta Part A, 2009, 72, 766-771
06.Rapid determination of gatifloxacin in biological samples and pharmaceutical products using europium-sensitized fluorescence spectrophotometry
Luminescence, 2008, 23, 7-13
05.Quantification of protein based on single-molecule counting by total internal reflection fluorescence microscopy with adsorption equilibrium
Analytica Chimica Acta, 2007, 590, 104-109
04.Fluorimetric Determination of Europium Based on the Co-fluorescence System Eu-Y-Sparfloxacin-SDS
Mikrochimica Acta, 2004, 144, 29-34
03.Determination of trace europium based on a new fluorescence system of europium-sparfloxacin-1,10-phenanthroline-sodium dodecyl sulfate
Analytical Bioanalytical Chemstry, 2003, 377(4), 681-684
02.Spectrofluorimetric determination of trace amounts terbium with 4-chlorsalicylic acid, EDTA, and cetyltrimethylammonium bromide
Analytical Sciences, 2003, 19, 923-925
01.Study of the absorption spectra of the 4f electron transitions of the praseodymium complex with ciprofloxacin and its analytical application



【科研项目】

01.国家基金面上项目:基于功能化石墨烯的循环肿瘤细胞分选与活细胞原位荧光成像研究(No.21675100),2017.01-2020.12,主持

02.国家基金面上项目:基于DNA催化自组装的模块化荧光生物传感技术及其生物医学应用研究 (No.21475077),2015.01-2018.12,主持

03.国家基金面上项目:基于结构DNA纳米材料的生物单分子荧光成像技术研究 (No.21175081),2012.01-2015.12,主持

04.国家基金面上项目:全内反射荧光显微术检测单个生物大分子 (No.20775043),2008.01-2010.12,主持

05.省基金重点项目:量子点结合荧光共振能量转移技术检测生物大分子 (Z2008B05),2009.01-2011.12,主持

06.省基金重点项目:单分子计数及细胞内单分子运动轨迹的观测 (Z2004B03 ),2005.01-2007.12,主持

【科研成果】

【所获专利】

【联合培养情况】

【拟招收研究生情况】

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